A Spectrophotometric Assay of ATP Synthesized by Sarcoplasmic Reticulum
نویسندگان
چکیده
منابع مشابه
Skeletal muscle sarcoplasmic reticulum glycogen status influences Ca uptake supported by endogenously synthesized ATP
Lees, Simon J., and Jay H. Williams. Skeletal muscle sarcoplasmic reticulum glycogen status influences Ca uptake supported by endogenously synthesized ATP. Am J Physiol Cell Physiol 286: C97–C104, 2004. First published September 10, 2003; 10.1152/ ajpcell.00188.2003.—The purpose of this investigation was to determine whether there is a link between sarcoplasmic reticulum (SR) glycogen status an...
متن کاملSkeletal muscle sarcoplasmic reticulum glycogen status influences Ca2+ uptake supported by endogenously synthesized ATP.
The purpose of this investigation was to determine whether there is a link between sarcoplasmic reticulum (SR) glycogen status and SR Ca2+ handling. In this investigation, skeletal muscle SR was purified from female Sprague-Dawley rats (200-250 g). Glycogen was extracted from the SR purified from one hindlimb, whereas the SR purified from the contralateral limb served as control. Before removal...
متن کاملMagnesium permeability of sarcoplasmic reticulum. Mg2+ is not countertransported during ATP-dependent Ca2+ uptake by sarcoplasmic reticulum.
Magnesium transport across sarcoplasmic reticulum (SR) vesicles was investigated in reaction mixtures of various composition using antipyrylazo III or arsenazo I to monitor extravesicular free Mg2+. The half-time of passive Mg2+ efflux from Mg2+-loaded SR was 100 s in 100 mM KCl, 150 S in 100 mM K gluconate, and 370 S in either 100 mM Tris methanesulfonate or 200 mM sucrose solutions. The conce...
متن کاملCa2+-dependent effect of ATP on spin-labeled sarcoplasmic reticulum.
Vesicular fragments of sarcoplasmic reticulum (SR) were labeled with the --SH-directed spin label 2,2,6,6-tetra-methyl,4-amino(N-iodoacetamide). Colorimetric titrations of the remaining --SH residues and determinations of unbound spin label indicated that primarily 3 residues/enzyme molecule were labeled under saturating conditions. This labeling was accompanied by minimal losses in activity, p...
متن کاملProtonation of the sarcoplasmic reticulum Ca-ATPase during ATP hydrolysis.
pH changes of the reaction medium (pH 6.35) following addition of MgATP were determined at 4 degrees C with sarcoplasmic reticulum vesicles in the presence of 0.1 mM CaCl2 and 5 mM MgCl2. With intact vesicles, a pronounced acidification following a slight alkalinization was induced by MgATP, indicating H+ ejection from vesicles. This agrees with previous observations by several investigators th...
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ژورنال
عنوان ژورنال: Australian Journal of Biological Sciences
سال: 1978
ISSN: 0004-9417
DOI: 10.1071/bi9780021